NON-REDUCING ALKALINE SOLUBILIZATION AND RAPID ON-COLUMN REFOLDING OF RECOMBINANT PRION PROTEIN
نویسندگان
چکیده
منابع مشابه
A comparative analysis of rapid methods for purification and refolding of recombinant bovine prion protein.
Bacterially-produced recombinant prion protein (rPrP) is a frequently used model system for the study of the properties of wild-type and mutant prion proteins by biochemical and biophysical approaches. A range of approaches have been developed for the purification and refolding of untagged rPrP expressed as inclusion bodies in Escherichia coli, including refolding by dialysis and simultaneous o...
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ژورنال
عنوان ژورنال: Preparative Biochemistry and Biotechnology
سال: 2012
ISSN: 1082-6068,1532-2297
DOI: 10.1080/10826068.2011.564256